Journal: Molecular medicine reports

Article Title: Swimming training promotes angiogenesis of endothelial progenitor cells by upregulating IGF1 expression and activating the PI3K/AKT pathway in type 2 diabetic rats.

doi: 10.3892/mmr.2024.13361

Figure Lengend Snippet: Figure 2. Morphology and identification of EPCs. (A) Characterization of EPCs from rats grown in vitro (magnification, x100). (B) Immunofluorescence staining with Dil‑ac‑LDL and/or FITC‑UEA‑I (scale bar, 20 µm). (C) Fluorescence microscopy detection of phenotypes CD31, CD34 and CD133 (scale bar, 20 µm). EPC, endothelial progenitor cell; Dil‑ac‑LDL, 1,1'‑dioctadecyl‑3,3,3', 3'‑tetramethylindocarbocyanine perchlorate‑labeled acetylated low‑density lipoprotein; FITC‑UEA‑I, fluorescein isothiocyanate‑conjugated Ulex europaeus agglutinin‑I.

Article Snippet: The primary antibodies were: CD31 (1:100; Proteintech Group; cat. no. CL488‐66065), CD34 (1:100; BIOSS; cat. no. bsm‐41197M) and CD133 (1:100; Proteintech Group; cat. no. 18470‐1‐AP).

Techniques: In Vitro, Immunofluorescence, Staining, Fluorescence, Microscopy

Journal: International journal of cardiology

Article Title: Enhanced external counterpulsation inhibits endothelial apoptosis via modulation of BIRC2 and Apaf-1 genes in porcine hypercholesterolemia.

doi: 10.1016/j.ijcard.2013.11.033

Figure Lengend Snippet: Fig. 2. Isolation and identification of vascular endothelial cells from the aortic endothelium with collagenase. Representative photomicrographs (×400) of isolated cells treated histocytochemically with CD31 antibody and incubated with DiI-Ac-LDL. Cells with cytomembrane and cytoplasmic staining of amber color indicate vascular endothelial cells (black arrow), and cells with red fluorescence were identified as viable vascular endothelial cells (white arrow), which consisted of the majority of total treated cells, indicating a successful en- dothelial cell isolation from aortic endothelium.

Article Snippet: Then mouse polyclonal CD31 antibody (at 1:100 dilution; Boster Biological Technology, Inc, Wuhan, China) was applied and incubated at 37 °C for 1 h, then stained with SABC, enhanced by DAB, dehydrated in a graded ethanol series, and followed by dimethylbenzene treatment to improve transparency, and finally mounted with neutral gum.

Techniques: Isolation, Incubation, Staining, Cell Isolation

Journal: Cells

Article Title: NFAT5/TonEBP Limits Pulmonary Vascular Resistance in the Hypoxic Lung by Controlling Mitochondrial Reactive Oxygen Species Generation in Arterial Smooth Muscle Cells

doi: 10.3390/cells10123293

Figure Lengend Snippet: Immunofluorescence-based analysis of SMC coverage of pulmonary arteries. Vibratome sections of lungs from hypoxia/normoxia-exposed Nfat5 (SMC)−/− and Nfat5 fl/fl mice were processed to detect CD31 (green) and αSMA (red) by immunofluorescence-based techniques (scale bars: 100 µm). Image stacks of confocal images were morphometrically evaluated to determine the coverage of arterial segments with different caliber comprising the following groups: 7–10 µm, 11–15 µm, 16–20 µm, and 21–25 µm (n.s.—not significant, * p < 0.05, *** p < 0.001 as indicated, n = 5–10).

Article Snippet: Subsequently, primary antibodies in PBS/5% donkey serum/0.5% Triton X100 (mouse anti-NFAT5 (direct label Alexa 546), sc-398171, Santa Cruz Biotechnology, Heidelberg, Germany, dilution: 1:100; goat anti-CD31, AF3628, biotechne, dilution 1:200; anti αSMA-Cy3, C6198 Sigma, dilution 1:700) were added for 24 h (4 °C).

Techniques: Immunofluorescence